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Soil sampling
Sampling (0-5 cm depth) was performed in two representative sites in Estarreja municipality:
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Contaminated soil (Vala de São Filipe)
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Uncontaminated soil (Salreu)
Approach
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Baseline characterization (pH, EC, OrgC, microbial biomass, soil respiration)
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Microbial in-vitro cultivation
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Biorreactor operation
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Aerobic fed-batch operation
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Assessment of biodegradative performance: glucose feeding
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Assessment of stability under toxic conditions: glucose replaced by toluene
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Addition of hardwood feedstock biochar as biofilm growing carrier
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Landfarming assay:
Some key results
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