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Soil sampling

Sampling (0-5 cm depth) was performed in two representative sites in Estarreja municipality:

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  • Contaminated soil (Vala de São Filipe)

  • Uncontaminated soil (Salreu)

Approach

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  • Baseline characterization (pH, EC, OrgC, microbial biomass, soil respiration)

  • Microbial in-vitro cultivation

  • Biorreactor operation

    • Aerobic fed-batch operation

    • Assessment of biodegradative performance: glucose feeding

    • Assessment of stability under toxic conditions: glucose replaced by toluene

    • Addition of hardwood feedstock biochar as biofilm growing carrier

  • Landfarming assay:

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Some key results

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